Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

  • Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
  • Treg cells can be expanded with Dynabeads® CD3/CD28 Treg Expander and remain
    Foxp3+.
  • Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology

The isolation procedure includes the following steps:
  1. Negative isolation of untouched human CD4+ T-cells from PBMC.
  2. Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
  3. Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD®.

Starting sample: PBMC