- High purity, recovery and viability of human untouched CD4+ T cells
- Easy to use and to scale up
- No columns required
Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended