Use this kit to isolate pure and viable untouched CD8+ T-cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD8+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended.

Starting Sample:
PBMC