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Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´-5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. • Fidelity – Greater than six times higher fidelity than Taq DNA polymerase • Amplicon Size – Amplification of fragments up to 15 kb (Figure 1) • Convenience – Room temperature reaction assembly • Applications – Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit Definition: One unit of Platinum® Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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