Grace's Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. One supplemented, Grace's Unsupplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Gibco® Grace's Unsupplemented Insect Medium can be used during transfection of insect cells, or for protein labeling studies (with methionine). Life Technologies offers a variety of Gibco® Media options for a range of insect cell culture applications.
The complete formulation is available. Grace's Unsupplemented Insect Medium does not contain lactalbumin hydrolysate and yeastolate commonly found in the supplemented version.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Grace's Unsupplemented Insect Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.

Grace's Unsupplemented Insect Medium requires supplementation with 10% Fetal Bovine Serum (FBS), 3.33 g/L lactalbumin hydrolysate and 3.33 g/L yeastolate before use as a growth medium. Grace's Unsupplemented Insect Medium requires ambient CO₂ in order to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).