HIV-1 glycoprotein 120 (gp120, fragment of env), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the HIV-1 surface glycoprotein gp120 (Ala 29-Glu 503), which is cleaved from the full length envelope glycoprotein gp160, fused to a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD4-His at 2 µg/well can bind biotinylated HIV-1 GP120 with a linear range of 0.078-1.25 µg/ml.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.