Copy and paste this HTML to embed a link in your website
Paste link in email or IM
The ThermoScript™ RT-PCR System is designed for the detection and analysis of RNA in a two-step process. ThermoScript™ reverse transcriptase (RT) is used to generate full-length cDNA transcripts using either total RNA or poly(A)+ RNA. PCR amplification is then performed using Platinum® Taq DNA Polymerase High Fidelity. • cDNA Yield—Low RNase H activity of ThermoScript™ RT maximizes first-strand cDNA yield • Thermostability—ThermoScript™ RT is active at temperatures up to 70°C • Fidelity—Platinum® Taq DNA Polymerase High Fidelity offers 6 times the fidelity of Taq DNA Polymerase alone • Amplicon Size—Platinum® Taq DNA Polymerase High Fidelity is ideal for templates from 100 bp to 15 kb (Figure 1)
High-Yield First Strand Synthesis ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR of your most highly structured, difficult RNA templates. ThermoScript™ RT-PCR Systems are designed to read through secondary structures and to improve priming specificity. Priming may be performed using oligo(dT), or random primers (both included), or a gene-specific primer. In conjunction with PCR, the system can be used to detect the presence of rare messages, or to quantitate the amount of specific mRNAs from small numbers of cells.
High-Fidelity PCR Amplification Platinum® Taq DNA Polymerase High Fidelity is ideal for the amplification of DNA fragments, when high yields and robust amplification are required. Fidelity is maximized through the use of a mixture of Platinum® Taq DNA Polymerase and the proofreading (3’-5’ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.