Dynabeads® Human T-Activator CD3/CD28 is for the activation and expansion of human T-cells for research purposes.With Dynabeads® Human T-Activator CD3/CD28 you get:
• Activation of T-cells without the need of feeder cells
• Activated cells that retain in vivo
• Maximal reproducibility without contamination by soluble antibodies or mitogens
• From 100- to 1000-fold T-cell expansionHow Dynabeads® Human T-Activator CD3/CD28 works:
Dynabeads® Human T-Activator CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet
For expansion of antigen specific T-cells from T-cell clones or T-cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137
Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T-cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. For an overview over the different Dynabeads® available for cell isolation, look here
The activated T-cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T-cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
For research use only. Not for use in diagnostic procedures.