Plant DNAzol® Reagent is a ready-to-use organic reagent formulated for the isolation of high quality genomic DNA from a variety of plant samples.
• Permits use on broad spectrum plant material
• Rapid isolation of genomic DNARNA Hydrolysis for Efficient Isolation of Genomic DNA from a Variety of Sample Sources
Plant DNAzol® Reagent can be used for the efficient isolation of genomic DNA from a variety of plant tissues such as leaf, seed stem, root, and callus. The Plant DNAzol® procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. Isolate Genomic Plant DNA in About an Hour
In the Plant DNAzol® procedure, plant samples are pulverized in liquid nitrogen or homogenized, and genomic DNA is extracted from the homogenate with the Plant DNAzol® Reagent. Following extraction, plant debris is removed by centrifugation and DNA is precipitated from the supernatant with ethanol, and solubilized with TE buffer (pH 8.0)
. The entire procedure can be completed in approximately 60-70 minutes. Comparable Yield in Less Time and with Fewer Reagents
Unlike traditional methods for plant DNA extraction (e.g., CTAB), Plant DNAzol® is quicker, does not require RNase A to remove RNA from genomic DNA preparations, and in the majority of tissues, does not require phenol. Furthermore, use of Plant DNAzol® generates yields from plant tissue that are comparable with the traditional CTAB method. Isolated DNA can be used for a Number of Downstream Applications:
• Amplified Fragment Length Polymorphism (AFLP)
• Restriction Fragment Length Polymorphism (RFLP)
• Southern Blotting
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.