Neurobasal®-A Medium is a basal medium formulated to meet the special cell culture requirements of post-natal and adult brain neuronal cells when used with GIBCO® B-27® Supplement. Neurobasal®-A Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal®-A Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.


Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with either a serum-free supplement (such as B-27® Supplement or N-2 Supplement ), or with serum and 0.5 mM L-glutamine or GlutaMAX™-I Supplement . For initial plating, you should add 25 µM glutamic acid.

Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination. The osmolality of Neurobasal®-A was demonstrated to be optimal for postnatal and adult CNS neurons.

Neurobasal®-A Medium contains Phenol Red. If Phenol Red will interfere with your applications, we also provide Neurobasal®-A Medium Minus Phenol Red.

Neurobasal®-A Medium is optimized for post-natal and adult brain neurons. For growing pre-natal and embryonic neurons, we provide Neurobasal® Medium.

See the entire Neurobasal® product line.

Learn more about neural cell culture products from Invitrogen at www.invitrogen.com/neuralculture.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.