Copy and paste this HTML to embed a link in your website
Paste link in email or IM
Defined Keratinocyte-SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers (1,2). The growth-promoting activity of BPE has been replaced by the inclusion of growth promoting agents which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM) allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.
Note: This formulation contains some human and animal origin components.
Note: A Defined Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative. Defined Keratinocyte-SFM consists of a basal liquid medium and frozen supplement which is added at the time of use. The fully supplemented medium has a shelf-life of 10 weeks under normal use conditions. Product performance is confirmed by evaluation of human primary keratinocyte growth.
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.