Exosome – Human CD63 Isolation/Detection enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.
• Obtain highly pure CD63+ exosomes
• "See" your sample during handling
• Easily scalable protocol
• Detect exosomes via flow cytometry, in less than 1 hour
Isolation and detection of exosomes has been a tedious, non-specific, and difficult process. Exosome – Human CD63 Isolation/Detection (from cell culture media) utilizes the well-known Dynabeads® magnetic separation technology, allowing you to easily purify pre-enriched CD63+ exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting.
Detect by Flow Cytometry
Because free exosomes alone are too small to be detected by flow cytometry, one of the major advantages of employing magnetic separation technology is that the purified, bead-bound exosomes can be easily visualized by flow cytometry. The monodispersed and relatively large Dynabeads® (4.5 µm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour.
"See" Your Sample
Not only are the superparamagnetic Dynabeads® known for their sensitivity, reproducibility, and stability, but the magnetic handling also allows you to "see" your sample due to the light brown color of the beads. When the sample tube is placed on the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and purification. In addition, the sample and bead volume can be easily scaled up or down according to your sample size or downstream applications.
Good Mixing is Critical
For successful exosome isolation it is important to use a mixer that tilts and rotates to ensure that the beads do not settle in the tube. Avoid end-over end rotation for small sample volumes (e.g., 100 µl). Please see user manual below for more guidelines.