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Express Five® SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 16 mM L-glutamine before use. Gibco® Express Five® SFM features:
• Superior long-term suspension growth of High Five™ cells • Protein-free, serum-free formulation • Optimized for recombinant protein production • Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System
Superior growth of High Five™ cells Gibco® Express Five® SFM provides peak cell densities of up to 6 x 106 viable cells per mL in shaker cultures. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® Express Five® SFM, usually without any further adaptation (see figure). Cells usually require adaptation from serum-containing formulations.
Protein-free, serum-free formulation Gibco® Express Five® SFM is a protein-free and serum-free formulation, allowing for easier purification of your protein of interest.
Optimized for recombinant protein expression Gibco® Express Five® SFM is optimized for high protein production using the Baculovirus Expression Vector System (BEVS). Using BEVS, we routinely achieve rβ-galactosidase protein expression of up to 1.7 U/cell, a significant improvement over other media (see figure)
Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System Protein production using Gibco® Express Five® SFM can be scaled up in bioreactors such as the 5 L CelliGen® bioreactor and the WAVE Bioreactor® System. The appropriate impeller or rocking speed and seeding density should be optimized for each system. Depending on the bioreactor design, it may be necessary to supplement Gibco® Express Five® SFM with additional Pluronic® F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system Gibco® Express Five® SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
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