The Elongase® Amplification System is ideal for amplifying DNA fragments up to 30 kb from purified DNA or crude samples (1). The enzyme mix is an optimized mixture of recombinant thermostable DNA polymerases,
Taq DNA polymerase, and the proofreading (3'-5' exonuclease activity) enzyme
Pyrococcus species
GB-D polymerase (Figure 1). Elongase® Amplification System and Enzyme Mix provides:
- Five-times higher fidelity than Taq DNA polymerase alone
- A buffer system that allows amplification of simple and complex genomic targets
- Amplification of long PCR templates or cDNA templates in RT-PCR applications (2)
