Retinol binding protein 4, plasma (RBP4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing functional assays. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding human RBP4 (NP_006735.2), including the signal sequence (Met 1-Leu 201), fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching oftryptophan fluorescence in RBP4. Recombinant human RBP4 can bind >1.0 µM all-trans retinoic acid .

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.