Cadherin 1, type 1, E-cadherin (epithelial) (CDH1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CDH1 precusor (NP_004351.1) (Met 1-Ile 707) fused to the polyhistidine tag at the C-terminus.
Activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells . When cells are added to CDH1/ECAD coated plates (1.5 µg/ml, 100 µl/well), approximately 60 %-80 % will adhere specifically .
Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Expiration Date: Expires one year from date of receipt when stored as instructed.
This protein is manufactured by SINO Biological.