Interleukin 1 receptor, type I (IL1R1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It is suitable for use as protein standard or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 332) of human IL1R1 (NP_000868.1) fused to the human IgG1 Fc region at the C-terminus.

Activity:
Measured by its binding ability in a functional ELISA:
1. Immobilized human IL1B at 20 µg/ml (100 µl/well) can bind human IL1R1-Fc with a linear range of 6.4-160 ng/ml.
2. Immobilized human IL1B-His at 10 µg/ml (100 µl/well) can bind human IL1R1-Fc. The ED₅₀ of human IL1R1-Fc is 0.198 µg/ml.
3. Immobilized human IL1B at 10 µg/ml (100 µl/well) can bind human IL1R1-Fc. The ED₅₀ of human IL1R1-Fc is 0.48 µg/ml.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.