Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative).Applications:
Immunoglobulin affinity purification and Immunoprecipitation.
Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:
- all four subclasses of human IgG
- mouse IgG
- rabbit IgG
- guinea pig IgG
- rat IgG
- goat IgG
- sheep IgG
- cow IgG
- horse IgG
Recombinant protein G does not bind
to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly
to IgG from dog.