Alanyl Amonipeptidase (ANPEP), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the metalloprotease domain (Lys 69-Lys 967, 603 Met/Ile) of the mature form of human ANPEP (NP_001141.2) with the signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC) . The specific activity is >2,500 pmoles/min/ µg

Formulation: Lyophilized in 25mM MES, 0.15 M NaCl, pH 6.5, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.